Diversigen offers a variety of high-throughput metagenomic services for research, clinical, (pre) clinical, environmental, and industrial samples. We provide consulting services for study design, sample handling, extraction, sequencing, data analysis, and data interpretation. Our experience places us at the forefront of the microbiome field. We provide fast and reliable turnaround of high quality data, competitive pricing, and personalized customer service. Our metagenomic services include:
16S rRNA GENE SEQUENCING and qPCR
Provides a common and reliable method for the characterization of the bacterial community associated with a given sample. The analysis for 16S includes phylogenetic or taxonomic classification, alpha and beta diversity comparisons, and cluster analyses. We are able to achieve accurate bacterial classification from isolated bacterial colonies or a mixed sample from any source.
ITS Sequencing and qPCR
Provides a common and reliable method for the characterization of fungi. We are able to achieve accurate taxonomic classification from isolated fungi or other complex samples.
A qPCR-based quantification approach for microbial or fungal absolute abundance and load profiling. Absolute quantification can be paired with our sequencing approaches to estimate bacterial or fungal counts and convert relative abundances to absolute quantities.
METAGENOMIC (WHOLE GENOME SHOTGUN) SEQUENCING
This service is a powerful method for detection, characterization, and quantification of microbial species. We provide reliable taxonomic classification, often to the species or strain level. We also perform gene content assessment for genes of interest or categories of interest and can assemble genomes through the alignment of contigs and scaffold reads.
We offer both Shallow and Deep Sequencing – Contact us at firstname.lastname@example.org to learn more about their respective applications.
SINGLE ORGANISM GENOME SEQUENCING
This is an excellent tool for assembling complete genomes de novo or by mapping to reference genomes. This service is applicable to bacterial and viral genomes.
VIRAL METAGENOMIC SEQUENCING
Diversigen offers end-to-end viral metagenomic sequencing capable of capturing DNA and RNA viruses simultaneously in simple or complex samples. This approach, which includes total nucleic acid extraction, cDNA generation, sequencing, and informatics, detects eukaryotic viruses and bacteriophage (prokaryotic viruses), including human health-relevant viruses.
Our novel viral classification pipeline performs searches in both the DNA and protein space to provide increased sensitivity and specificity. Our viral analysis pipeline has been used to successfully detect SARS-CoV-2, Orthomyxoviruses (including influenza), Hepacivirus, Human Immunodeficiency Virus, and Enterovirus, among others.
Our sequencing platforms include:
- Illumina NovaSeq
- Illumina HiSeq
- Illumina MiSeq
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Diversigen provides data for research and detection purposes only and is not a substitute for diagnostic or actionable telemetry in humans. Information provided with implications for human health requires a clinical and FDA approved validation.